md-medicaldata

Authors

 

Jelena Ćurčić¹, Dragana Danilov<sup>2

1</sup>Univerzitet u Novom Sadu, Medicinski fakultet, Hajduk Veljkova 1, Novi Sad, Srbija
²Univerzitet u Novom Sadu, Prirodnomatematički fakultet, Departman za biologiju i ekologiju, Trg Dositeja Obradovića 2, Novi Sad, Srbija

 

UDK: 618.15-002-078

The paper was received / Rad primljen: 01.04.2023

Accepted / Rad prihvaćen: 21.05.2023.

 

Correspondence to:

Prof. dr Dušan Lalošević,
Katedra za histologiju i embriologiju
Medicinski fakultet Novi Sad
Hajduk Veljkova 3
Univerzitet u Novom Sadu, Srbija
Tel: +381 64 5060885
e-mail: dusan.lalosevic@mf.uns.ac.rs
e-mail: jelenacurcic.98@gmail.com
e-mail: injacd@yahoo.com

 

 

Sažetak

 

 

Uvod: Najčešća metoda ispitivanja citoloških i mikrobioloških elemenata vaginalnog brisa u rutinskoj praksi je direktno mikroskopsko ispitivanje nativnog ili bojenog preparata. Cilj rada: Ovladavanje osnovnim tehnikama mikroskopskog pregleda vaginalnog brisa i upoređivanje vrednosti nativnog u odnosu na bojeni preparat vaginalnog brisa u rutinskoj citološkoj i mikrobiološkoj dijagnostici. Materijal i metode: Vaginalni brisevi prikupljeni su od 45 žena. Prva grupa imala je 30 ispitanica, dok je druga od 15 ciljano pregledana na trihomonijazu. Odmah posle uzimanja bris je u originalnoj epruveti potopljen u sterilni fiziološki rastvor i transportovan do laboratorije gde je u roku od dva sata urađen mikroskopski pregled nativnog preparata. Svaki nativni preparat je posle pregleda osušen, fiksiran, obojen metodom po Papanikolau i mikroskopski analiziran na istom uveličanju. Rezultati: Mikroskopskim pregledima nativnih i obojenih preparata viđene su ćelije svih slojeva vaginalnog pločastoslojevitog epitela, leukociti i razni mikroorganizmi kao što su Doderlajnova flora, nespecifična aerobna flora i anaerobna flora bakterijske vaginoze sa tzv. “clue” ćelijama. U jednom slučaju videli smo prisustvo filamentoznih bacila iz grupe Leptotrix, u drugom Trichomonas. Uspeli smo da identifikujemo koilocite kao znak  infekcije humanim papiloma virusima (HPV). Svi ovi elementi vaginalnih briseva viđeni su u obe vrste mikroskopskih preparata, tako da uporedna analiza nije pokazala značajnu razliku između nativnih i obojenih preparata. Zaključak: S obzirom na sličnu dijagnostičku vrednost obe metode, nativni preparat ima prednost u odnosu na bojeni zbog svoje jednostavnosti, brzo dobijenih rezultata i mogućeg brzog započinjanja adekvatne terapije. Razlog što se nativni preparat ređe koristi u rutinskoj praksi je potreba za brzim transportom do mikroskopičara, što se može prevazići relativno kratkom obukom ginekologa da samostalno analiziraju briseve neposredno posle uzimanja.

 

Ključne reči:

vaginalni bris; nativni preparat; bojeni preparat

 

 

 

Abstract

 

Introduction: The most common method of examination of cytological and microbiological elements of vaginal smear in the routine practice is direct microscopic examination of wet mount or stained smear. The Aim: Mastering the basic techniques of microscopic examination of vaginal swabs and comparing the value of wet mount versus dyed slides of vaginal swabs in the routine cytological and microbiological diagnostics. Material and Methods: Vaginal swabs  were collected from 45 women. The first group was consisted of 30 subjects whose swabs were collected during routine gynecological examination. The second group of 15 subjects was specifically targeted for trichomoniasis. Immidiately after the collection, the swab was immersed in a sterile physiological sollution in the original test tube and transported to the laboratory, where the microscopical examination of the vaginal wet mount was preformed within two hours. After microscopic examination, each slide was dried, fixed, stained with Papanocolau stain method and microscopically analyzed at the same magnification. Results: Microscopic examination of wet mount and stained vaginal smears showed us cells of all layers of vaginal squamous epithelium, leukocytes and various microorganisms such as Döderlein bacilli, non-specific aerobic flora and anaerobic flora of bacterial vaginosis with the so-called "clue" cells. In one case we saw the presence of filamentous bacilli from the Leptotrix group, in the other Trichomonas vaginalis. We were able to identify koilocytes as a sign of human papillomavirus (HPV) infection. All these elements of vaginal swabs were seen in both types of the microscopic preparations, so the comparative analysis showed no significant difference between the wet mount and the stained vaginal smears. Conclusion: Given the similar diagnostic value of both methods, the wet mount has an advantage over the stained one due to its simplicity, quickly obtained results and possible rapid initiation of adequate therapy. The reason why the wet mount is less often used in the routine practice is the need for rapid transport to the microscopist, which can be overcome by relatively short training of gynecologists to independently analyze swabs immediately after the collection.

 

Key words:

vaginal smear; wet mount; stained smear

 

 

 

 

References:

1. Lalošević D i sar. Mikroskopska laboratorijska tehnika u medicini. Univerzitet u Novom Sadu, Medicinski fakultet, Katedra za histologiju i embriologiju 2005; Novi Sad.
2. Lash AF, Kaplan B. A Study of Doderleins Vaginal Bacillus. The Journal of Infectious Diseases 1926; 38(4): 333-40.
3. Diamantis A, Magiorkinis E, Koutselini H.  50 Years After the Death of George Nicholas Papanikolaou (1883-1962): Evaluation of His Scientific Work. Acta med-hist Adriat 2014; 12(1):181-8.
4. Tomanović S, Stojković M. Izmenjeni sastav vaginalnog mikrobioma-primeri iz prakse. Zbornik radova 63. Ginekološko akušerske nedelje SLD, 2019; Beograd: 171-3.
5. Vrzić-Petronijević S. Vaginalni mikrobiom i reproduktivno zdravlje žene. Zbornik radova 63. Ginekološko akušerske nedelje SLD, 2019; Beograd: 169-70.
6. Đukić S. Normalni i izmenjeni vaginalni mikrobiom. Zbornik radova 63. Ginekološko akušerske nedelje SLD, 2019; Beograd:167-8. 
7. Sherrard J, Wilson J, Donders G, Mendling W, Jensen JS. 2018 European (IUSTI/WHO) International Union against sexually transmitted infections (IUSTI) and World Health Organisation (WHO) guideline on the management of vaginal discharge. International Journal of STD&AIDS 2018; 29(13): 1258-72.
8. Mylonas I, Bergauer F. Diagnosis of Vaginal Discharge by Wet Mount Microscopy: A Simple and Underrated Method. Obstet Gynecol Surv 2011; 66(6): 359-68.
9. Donders GG, Marconi C, Bellen G, Donders F, Michels T. Effect of Short Training on Vaginal Fluid Microscopy (Wet Mount) Learning. J Lower Gen Tract Dis 2015; 2 (19): 165-9.
   

PDF: 10-Ćurčić J. and Injac D. MD-Medical Data 2023;15(1-2) 047-051.pdf