md-medicaldata

Authors

 

Dušan Lalošević¹, Srđan Stankov¹, Veselin Bojat², Sava Lazić³, Tamaš Petrović³, Dejan Vidanović⁴, Dragana Vujin¹, Verica Simin¹, Vesna Lalošević<sup>5

1</sup>Pasterov zavod Novi Sad
²Dom zdravlja Novi Sad
³Naučni institut za veterinarstvo “Novi Sad”, Novi Sad
⁴Veterinarski specijalistički institut “Kraljevo”, Kraljevo
⁵Poljoprivredni fakultet, Univerzitet Novi Sad

 

UDK: 578.834(497.113)"2021

The paper was received / Rad primljen: 22.06.2021.

Accepted / Rad prihvaćen: 30.06.2021.

 

Correspondence to:

Prof. dr Dušan Lalošević,
Medicinski fakultet Univerziteta u Novom Sadu i Pasterov zavod Novi Sad,
Hajduk Veljkova 1, 21000 Novi Sad,
e-mail: dusan.lalosevic@mf.uns.ac.rs

 

 

Abstract

 

 

Virus isolation on Vero cell culture was successful from a single and a pool of 4 swabs of patients positive in a rapid antigen test for SARS-CoV-2. Genome RNA sequencing of both SARS-CoV-2 isolates designed as Novi Sad 2021-1 and 2 (NS21-1; NS21-2) proved that they belong to the "British" strain. Adaptation of virus isolates to Vero culture was done in three consecutive passages, whereby the incubation was shortened from 6 days to 36 hours until the appearance of the first cytopathic effect (CPE). Viral harvest titers ranged from 4 to 7 logCCID50/mL. Inactivation of the isolates was successful with beta-propiolactone, 1: 1000, with preserved viral antigenicity.

 

 

 

Keywords:

SARS-CoV-2, Novi Sad, isolation, viral inactivation

 

 

 

Sažetak

Izolacija virusa na Vero ćelijskoj kulturi uspela je iz jednog pojedinačnog i pula od 4 brisa pacijenata pozitivnih u brzom antigenskom testu na SARS-CoV-2. Sekvenciranjem genoma oba izolata SARS-Cov-2 označenih kao Novi Sad 2021-1 i 2 (NS21-1; NS21-2) je utvrđeno da oba pripadaju “britanskom” soju. Adaptacija izolata virusa SARS-CoV-2 na Vero kulturu ćelija urađena je u tri serijske pasaže, pri čemu se inkubacija do pojave prvog citopatogenog efekta (CPE) skratila od 6 dana na 36 sati. Titrovi virusnih berbi kretali su se od 4-7 logCCID50/mL. Inaktivacija izolata uspela je beta-propiolaktonom, 1:1000, uz očuvanu antigenost virusa.

 

 

Ključne reči:

SARS-CoV-2, Novi Sad, izolacija, inaktivacija virusa

 

 

 

 

References:

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3. Lalošević D. Domaća proizvodnja seruma i vakcina. Medical Data 2020, 12(3):1.
4. Yao P, Zhang Y, Sun Y, et al. Isolation and growth characteristics of SARS-CoV-2 in Vero cell. Virologica Sinica (2020) 35:348–350. doi.org/10.1007/s12250-020-00241-2.
5. Harcourt J, Tamin A,Lu X, et al. Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient. bioRxiv journal, doi.org/10.1101/2020.03.02.972935.
   

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