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PRAĆENJE CMV VIREMIJE NAKON TRANSPLANTACIJE MATIČNIH ĆELIJA HEMATOPOEZE /

MONITORING OF CMV VIRAL LOAD AFTER STEM CELL TRANSPLANTATION

Authors

 

Danijela Đurić-Petković1 , Elizabeta Ristanović1

1Vojnomedicinska akademija, Institut za mikrobiologiju, Beograd

 

UDK: 616.45-089-06(497.11)"2012/2016"


The paper was received / Rad primljen: 17.06.2020.

Accepted / Rad prihvaćen: 22.06.2020.

 


Correspondence to:


Danijela Đurić Petković
Vojnomedicinska akademija
Institut za mikrobiologiju
Crnotravska 17
Beograd
telefon 063 440-482
e-mail: danijeladjuricpetkovic@gmail.com

 

 

Sažetak

 

Citomegalovirus (CMV) je uzročnik brojnih komplikacija nakon transplantacije matičnih ćelija hematopoeze (TMĆH) kao što su pneumonija, retinitis, gastroenteritis, hepatitis, infekcije CNS. CMV je  jedan od najznačajnijih faktora  mortaliteta i morbiditeta nakon transplantacije matičnih ćelija. Za praćenje naših pacijenata koristili smo CMV DNK kvantitativni Real-time test lančane reakcije polimeraze. Naš cilj je bio utvrđivanje  viremije CMV kod pacijenata nakon TMĆH.                                     

 

 

 

Ključne reči:

Citomegalovirus, alogena transplantacija, viremija.

 

 

Abstract

 

Background/Aim. Cytomegalovirus (CMV) causes major complications after allogeneic stem cell transplantation (SCT) such as pneumonia, retinitis, gastroenteritis, hepatitis, CNS infections. CMV is still a major cause of morbidity and mortality after hematopoietic cell transplantation. For CMV DNA monitoring in the samples of our patients we used quantitative real-time polymerase chain reaction (PCR) assays. The aim was use to determine CMV loads in patients undergoing SCT. Methods: A total of 50 SCT patients were studied during a 100 days period after transplantation for the presence of CMV DNA. Polymerase chain reaction (PCR) CMV DNA was performed on 488 full blood samples using Gene Proof CMV DNA tests. Results: CMV viremia was detected in 27/50 (54%) of the patients. The median posttransplantation time for the first positive PCR result was 35,15 days after SCT. Detected viral CMV load was between 336 copies/ml and 27800 copies/ml. Conclusion. Early detection of CMV in blood is very important for preemptive therapy. It is better to prevent than to treat CMV disease and its possible consequences.

 

 

Key words:

Cytomegalovirus, allogeneic transplantation, viral load.

 

 

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PDF Đurić Petković D. and Ristanović E. • MD-Medical Data 2020;12(2): 087-089

 

 

 

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