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VARIJABILNOST MIKROSATELITSKIH MARKERA X HROMOZOMA I NJIHOVA PRIMENA U FORENZICI
GENETIC VARIABILITY OF X CHROMOSOME MICROSATELLITE MARKERS IN FORENSIC ANALYSIS

Authors

 

Dušan Vapa1, Igor Veselinović1, Radosav Radosavkić1, Nevena Veličković2, Milan Popović3

1Katedra za sudsku medicinu, Univerzitet u Novom Sadu, Medicinski fakultet,
2Departman za biologiju i ekologiju, Univerzitet u Novom Sadu Prirodno-matematički fakultet,
3Katedra za histologiju i embriologiju, Univerzitet u Novom Sadu, Medicinski Fakultet

 


Rad je primljen 24.07.2017. / Prihvaćen 01.08.2017.

 


Correspondence to


Doc. dr Dušan Vapa,
Univerzitet u Novom Sadu
Medicinski fakultet, Katedra za sudsku medicinu,
Hajduk Veljkova 3,
21 000 Novi Sad
e-mail: dusan.vapa@mf.uns.ac.rs

 

 

Sažetak

 

Kratki uzastopni ponovci predstavljaju klasu mikrosatelitskih segmenata DNK, rasprostranjenih širom genoma čoveka. Građeni su od uzastopno ponavljajućih sekvenci dužine 2-6 parova nukleotida. Zahvaljujući različitom broju ponavljanja repetitivne jedi­nice, većina mikrosatelitskih markera pokazuje visok stepen polimorfizma dužine, koji je moguće ispitati primenom tehnike lančane reakcije polimeraze. Pored utvrđivanja spor­nih srodničkih odnosa, analiza X hromozom mikrosatelitskih markera može se uspešno koristiti i u oblastima kriminalistike, humane identifikacije, populaciono-genetičkim i demografskim istraživanjima i dr. Cilj našeg istraživanja bila je izrada populacione studije, iz koje će se izračunati broj i frekvencija alela, struktura i frekvencija haplotipova, utvrditi vrednosti relevantnih statističkih parametara, i oceniti mogućnost primene analize X-STR markera u slučajevima iz oblasti medicinske kriminalistike, humane identifikacije i veštačenja spornih srodničkih odnosa u populaciji Vojvodine. Istraživanje je izvršeno u Centru za sudsku medicinu, toksikologiju i molekularnu genetiku, u Novom Sadu, u vidu prospektivne studije, na uzorku od 200 odraslih, međusobno nesrodnih osoba, oba pola. Izolacija DNK vršena je Chelex metodom, amplifikacija dobijenih uzoraka vršena je metodom PCR, uz korišćenje komercijalnog Mentype® Argus X-8 PCR Amplification Kit-a. Razdvajanje dobijenih fragmenata izvršeno je kapilarnom elektroforezom na aparatu ABI Prism 310 Genetic Analyzer. Za interpretaciju dobijenih rezultata korišten je GeneScan i Genotyper program, a za statističke analize programi Arlequin i GENEPOP. Dobijeni rezultati ukazuju da se ova metoda može uspešno primeniti u oblastima medicinske kriminalistike, humane identifikacije i veštačenja spornih srodničkih odnosa, a ujedno predstavljaju i bazu podataka za dalja istraživanja.

 

 

 

Ključne reči:

DNK; Mikrosatelitski markeri; X-STR; sudska medicina; forenzička genetika

 

 

Abstract

 

The aim of this research was to conduct a population study, which results will be used to calculate allele and haplotype frequencies, determine the value of relevant statistical parameters and assess the possibility of applying X-STR markers analysis in the fields of forensics, human identification and kinship testing. The study included 200 unrelated adults. DNA isolation was performed by Chelex method. DNA amplification was performed by PCR method, using commercial Mentype Argus X-12 PCR Amplification Kit. Separation and detection of fragments was obtained by capillary electrophoresis using GeneScan and Genotyper program. Statistical analysis of the results was performed using Arlequin and GENEPOP program. For visualization of interpopulation genetic distances POPTREE2 program was used. The results show that the analysis of X-STR markers can be successfully applied in the field of forensics, human identification and kinship testing in the population of Vojvodina, as well as to serve as a basis for further research in population genetic, anthropological, demographic and other scientific areas. According to authors current knowledge, the study of X chromosome microsatellite markers was never conducted in our country and these data represent the first population data of its kind in Serbia.

 

 

Key words:

X-Chromosome STRs; Argus X-12; Population data; Forensic genetics; Microsatellite repeats; Serbian population.

 

 

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UDK: 547.963.3:343.983
COBISS.SR-ID 245428236



PDF Vapa D. et al • MD-Medical Data 2017;9(3): 171-176

 

 

 

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