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COEXISTENCE OF SALMONELLA ENTERICA AND HUMAN EPITHELIAL CELLS-IN VITRO SYSTEM
KOEGZISTENCIJA SALMONELLA ENRERICA I HUMANIH EPITELIJALNIH ĆELIJA- IN VITRO SISTEM

Authors

 

Ksenija Durgo1, Jelena Miđić1, Ana Butorac1, Mario Cindrić2 and Višnja Bačun-Družina1
¹Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10000 Zagreb, Croatia
²Institute Rudjer Bošković, Bijenička 4, 10000 Zagreb, Croatia


 

• The paper was received on 15.09.2016. / Accepted on 22.09.2016

 

Correspondence to:
Ksenija Durgo,
Faculty of Food Technology and Biotechnology,
University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia,
E-mail: kdurgo@pbf.hr, ksenija974@gmail.com

 

Abstract

 

Salmonella entericais an ultimative human pathogen that causes various health problems. On the other hand, Lactobacillus plantarumhas numerous positive effects on human health. The objective of this study was to investigate the influence of enterobacteriaS. entericaLT21 on human laryngeal carcinoma cell line (HEp2). The ability of the adhesion of S. entericaLT21 as an independent culture and in combination with L. plantarum16.4 to HEp2 cells was tested as well as metabolic activity of S. entericaLT21 after adhesion to HEp2 cells. The protein content in HEp2 cells after the incubation with S. entericaLT21 was investigated and compared with the protein content in the untreated HEp2 cells. Adhesion results show better binding of S. entericaLT21 than L. plantarum16.4 when bacteria are mixed in a 1:1 and 10:1 ratios, respectively. Acetic and lactic acid in the sample of HEp2 cells incubated with S. entericaLT21 were detected, as well as lactic acid in a sample of HEp2 cells. Proteomic studies were also performed using tandem mass spectrometry analysis (MS/MS). Results imply that S. entericaLT21 affects the expression of proteins of HEp2 cells and moreover  HEp2 cells after incubation with S. entericaLT21 additionally expressed protein troponin T type 1.

 

 

Key words

S. enterica LT21, HEp2, adhesion, proteomics, metabolism

 

 

References

 

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UDK: 615.33.015.4:579.842.1/.8
618.146-006.6-085
COBISS.SR-ID 226163724



PDF Durgo K. et al • MD-Medical Data 2016;8(3): 165-167

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