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NOVI KORONA-VIRUS (SARS-CoV-2) IZOLATI NOVI SAD 2021, IDENTIFIKACIJA I INAKTIVACIJA /

NOVEL CORONAVIRUS (SARS-CoV-2) ISOLATES NOVI SAD 2021, IDENTIFICATION AND INACTIVATION

Authors

 

Dušan Lalošević1, Srđan Stankov1, Veselin Bojat2, Sava Lazić3, Tamaš Petrović3, Dejan Vidanović4, Dragana Vujin1, Verica Simin1, Vesna Lalošević5

1Pasterov zavod Novi Sad
2Dom zdravlja Novi Sad
3Naučni institut za veterinarstvo “Novi Sad”, Novi Sad
4Veterinarski specijalistički institut “Kraljevo”, Kraljevo
5Poljoprivredni fakultet, Univerzitet Novi Sad

 

UDK: 578.834(497.113)"2021


The paper was received / Rad primljen: 22.06.2021.

Accepted / Rad prihvaćen: 30.06.2021.

 


Correspondence to:


Prof. dr Dušan Lalošević,
Medicinski fakultet Univerziteta u Novom Sadu i Pasterov zavod Novi Sad,
Hajduk Veljkova 1, 21000 Novi Sad,
e-mail: dusan.lalosevic@mf.uns.ac.rs

 

 

Abstract

 

 

Virus isolation on Vero cell culture was successful from a single and a pool of 4 swabs of patients positive in a rapid antigen test for SARS-CoV-2. Genome RNA sequencing of both SARS-CoV-2 isolates designed as Novi Sad 2021-1 and 2 (NS21-1; NS21-2) proved that they belong to the "British" strain. Adaptation of virus isolates to Vero culture was done in three consecutive passages, whereby the incubation was shortened from 6 days to 36 hours until the appearance of the first cytopathic effect (CPE). Viral harvest titers ranged from 4 to 7 logCCID50/mL. Inactivation of the isolates was successful with beta-propiolactone, 1: 1000, with preserved viral antigenicity.

 

 

 

Keywords:

SARS-CoV-2, Novi Sad, isolation, viral inactivation

 

 

 

Sažetak


Izolacija virusa na Vero ćelijskoj kulturi uspela je iz jednog pojedinačnog i pula od 4 brisa pacijenata pozitivnih u brzom antigenskom testu na SARS-CoV-2. Sekvenciranjem genoma oba izolata SARS-Cov-2 označenih kao Novi Sad 2021-1 i 2 (NS21-1; NS21-2) je utvrđeno da oba pripadaju “britanskom” soju. Adaptacija izolata virusa SARS-CoV-2 na Vero kulturu ćelija urađena je u tri serijske pasaže, pri čemu se inkubacija do pojave prvog citopatogenog efekta (CPE) skratila od 6 dana na 36 sati. Titrovi virusnih berbi kretali su se od 4-7 logCCID50/mL. Inaktivacija izolata uspela je beta-propiolaktonom, 1:1000, uz očuvanu antigenost virusa.

 

 


Ključne reči:

SARS-CoV-2, Novi Sad, izolacija, inaktivacija virusa

 

 

 

 

References:

  1. Kolarević S, Micsinai A, Szántó-Egész R, et al. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. Science of the Total Environment 783 (2021) 146967.
  2. Ristić M, Nikolić N,Čabarkapa V, Turkulov V, Petrović V. Validation of the STANDARD Q COVID-19 Antigen test in Vojvodina, Serbia.PloS ONE (2021) 16(2):e0247606.https://doi.org/10.1371/journal.pone.0247606.
  3. Lalošević D. Domaća proizvodnja seruma i vakcina. Medical Data 2020, 12(3):1.
  4. Yao P, Zhang Y, Sun Y, et al. Isolation and growth characteristics of SARS-CoV-2 in Vero cell. Virologica Sinica (2020) 35:348–350. doi.org/10.1007/s12250-020-00241-2.
  5. Harcourt J, Tamin A,Lu X, et al. Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient. bioRxiv journal, doi.org/10.1101/2020.03.02.972935.

PDF00-MD-Vol 13 No 2 Jun 2021_Lalosevic et al.

 

 

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